Western Blotting

I could likely find an answer for you, if you post the problems you are having.

Or, try asking here if no one around here can help.
 
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In addition, there are scores of textbooks dealing with western blots (and half of the book is usually about trouble shooting). Or, check the manufacturers of the various western kits. They usually have very good protocols and usually a pretty good techinical support (if you use their product that is).
 
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