One question about RNA polymerase

Eagle9

Registered Senior Member
RNA polymerase is an enzyme that produces RNA during transcription. To do this the two strands of DNA must separate. During replication this is done by other enzyme-helicase:
The enzyme that separates the strands is known as helicase
http://www.austincc.edu/emeyerth/dnarep.htm
But as I know during transcription helicase is not used for separating DNA strands and this job is done by RNA polymerase itself (I speak about prokaryotes). RNA polymerase has got several subunits:
RNAP is a large molecule. The core enzyme has five subunits (~400 kDa):[5]
• β': The β' subunit is the largest subunit. The β' subunit contains part of the active center responsible for RNA synthesis and contains some of the determinants for non-sequence-specific interactions with DNA and nascent RNA.
• β: The β subunit is the second-largest subunit. The β subunit contains the rest of the active center responsible for RNA synthesis and contains the rest of the determinants for non-sequence-specific interactions with DNA and nascent RNA.
• αI and αII: The α subunit is the third-largest subunit and is present in two copies per molecule of RNAP, αI and αII. Each α subunit contains two domains: αNTD (N-Terminal domain) and αCTD (C-terminal domain). αNTD contains determinants for assembly of RNAP. αCTD (C-terminal domain) contains determinants for interaction with promoter DNA, making non-sequence-non-specific interactions at most promoters and sequence-specific interactions at upstream-element-containing promoters, and contains determinants for interactions with regulatory factors.
• ω: The ω subunit is the smallest subunit. The ω subunit facilitates assembly of RNAP and stabilizes assembled RNAP.
http://en.wikipedia.org/wiki/RNA_polymerase#RNA_polymerase_in_bacteria
But here is not written which subunit can separate two strands of DNA and I would like to know this. It is function of some subunits (β, β' or others) or is it a function of the whole polymerase? :rolleyes:
 
This different roles/utility of RNA polymerase is connected to the potential within water and configurational equilibrium. For cells to replicate they build up food reserves to drive the energy intensive synthesis. This accumulating food energy means accumulation of reduced carbon, which will increase the net potential of the water. This change in the water will change the surface tension between the water and organics making it possible for the DNA double helix to separate without helicase.
 
Back
Top