Eagle9
Registered Senior Member
RNA polymerase is an enzyme that produces RNA during transcription. To do this the two strands of DNA must separate. During replication this is done by other enzyme-helicase:
But as I know during transcription helicase is not used for separating DNA strands and this job is done by RNA polymerase itself (I speak about prokaryotes). RNA polymerase has got several subunits:
But here is not written which subunit can separate two strands of DNA and I would like to know this. It is function of some subunits (β, β' or others) or is it a function of the whole polymerase?
http://www.austincc.edu/emeyerth/dnarep.htmThe enzyme that separates the strands is known as helicase
But as I know during transcription helicase is not used for separating DNA strands and this job is done by RNA polymerase itself (I speak about prokaryotes). RNA polymerase has got several subunits:
http://en.wikipedia.org/wiki/RNA_polymerase#RNA_polymerase_in_bacteriaRNAP is a large molecule. The core enzyme has five subunits (~400 kDa):[5]
• β': The β' subunit is the largest subunit. The β' subunit contains part of the active center responsible for RNA synthesis and contains some of the determinants for non-sequence-specific interactions with DNA and nascent RNA.
• β: The β subunit is the second-largest subunit. The β subunit contains the rest of the active center responsible for RNA synthesis and contains the rest of the determinants for non-sequence-specific interactions with DNA and nascent RNA.
• αI and αII: The α subunit is the third-largest subunit and is present in two copies per molecule of RNAP, αI and αII. Each α subunit contains two domains: αNTD (N-Terminal domain) and αCTD (C-terminal domain). αNTD contains determinants for assembly of RNAP. αCTD (C-terminal domain) contains determinants for interaction with promoter DNA, making non-sequence-non-specific interactions at most promoters and sequence-specific interactions at upstream-element-containing promoters, and contains determinants for interactions with regulatory factors.
• ω: The ω subunit is the smallest subunit. The ω subunit facilitates assembly of RNAP and stabilizes assembled RNAP.
But here is not written which subunit can separate two strands of DNA and I would like to know this. It is function of some subunits (β, β' or others) or is it a function of the whole polymerase?