Lab glassware cleaning

[VentsyBG]

Can you tell me some protocols for cleaning of laboratory glassware after DNA/RNA extraction, PCR etc.

 

[Idle Mind]

Scrub with lots of soap, rinse well, and then autoclave.

 

[VentsyBG]

No need of ethanol or something like that? OK, thanks

 

[CharonZ]

Actually I wonder what glasswares are used for DNA/RNA works. Especially considering the fact that DNA binds wonderfully to glass. Or do you mean glasswares containing the relevant buffers and solutions? Anyway, if the glasswares are going to be used for sensitive works one should remember that tensides might harm you more than anything else. Often an additional rinsing with 5M HCl, followed by deionised water is recommended. Also for RNA works one should remember that mere autoclaving does not inactivate all RNAses. In that case baking for several hours at ~180°C is needed. Alternatively treatment with 0.1% DEPC is possible, followed by autoclaving (caution: it is toxic).
Also bear in mind that sometime autoclaving does not totally inactivate DNA. At least in a PCR reaction one can still get amplificates from autoclaved DNA (depending on the length of the product, of course).

 

[VentsyBG]

What I ment was the glassware for the buffers etc., not DNA or RNA.

 

[CharonZ]

If said solution is going to be in contact with RNA and DNA same rules apply

 

[VentsyBG]

OK, I will have it on my mind. Now I am going home, so have a nice day !