Discovery of Degenerate Genetic Code (question)

[genetictac]

Hello to all,


I am a student in Master1 of cellular and molecular biology. While looking for how the genetic code was discovered (and especially the notion of degeneracy), I came across a historical article from 1961 (General Nature of Genetic Code for Proteins, Crick et al.). I am trying to understand how the T4 rII system used in the experimental model in the article works. I found a good video in English explaining this but I am not sure I understand the difference between K and B cultures very well. Could someone please enlighten me? Here is the link to the video:

Have a nice day,

Jérome.

 

[origin]

We have to watch a hour long video just to get to your question? Couldn't you just flesh out your question a bit more here?

 

[genetictac]

We have to watch a hour long video just to get to your question? Couldn't you just flesh out your question a bit more here?

Hello Origin,

The portion of the video I am talking about is spreading from 1'40'' to 6'10'', so less than 5 minutes (the part about the T4 rII system that I don't really understand). I wanted to know what are the differences between the culture medium K and B... I don't understand how they differ at a cellular level, ones being "host" for recombination and other used just to know whether the rII gene is (non)-functional. Do you have any idea?

 

[Hercules Rockefeller]

I am trying to understand how the T4 rII system used in the experimental model in the article works.

Wow, you’re giving me flashbacks to my university genetics lectures! It’s been almost 30 years since I studied bacteriophage transduction of E.coli and how you can use it to determine genetic structure and gene order. It’s a very specific teaching method and a very specific question that I don’t have the time to re-learn in order to answer you. Sorry. A study session with your fellow classmates would be the best approach.