Dilution help

[marinerush]

So I'm currently growing 5 bacterial cell cultures. I start at the dilution of 10^-1 by adding 300 microliters of a media and 33 microliters of the bacterium. So when we do these dilutions fewer cells are developed. Is it because we are starting with fewer cells or is it because we are using less spent media?

 

[Hercules Rockefeller]

I am not sure I understand your question. Are you performing serial dilutions of a bacterial culture? If yes, then each diluted culture will exhibit a slower (log phase) growth rate than the culture before it as it has started with fewer bacterial cells.