Another plant Bio Question

[RateLimit]

I don't have a standard marker soooo without how do I:

Determine how much protein I have loaded into the gel

and

How do I quantitate the amount of protein in each of my sample on my western blot.

The latter question is without using the A260/A280.

Thanks in advance.

 

[river-wind]

arg, brain leak detected in sector 12. sorry dude, it's been just a second to long ago, I can't remeber......





edit:


basic gel electrophoresis

intro to protein detection

good luck!

 

[RateLimit]

I'm a girl!


But thanks, I figured it out.